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Juvenile hormone regulates extreme mandible growth in male stag beetles.%y ¥®ÂήɴÁªº¶Pº¸»X´xºÞ¤F¤½ÂΪº¤jÃEªº¦¨ªøg)9y>x Gotoh H, Cornette R, Koshikawa S, Okada Y, Lavine LC, Emlen DJ, Miura T.(5! ¤@°ï¦W¦rU ©©øÂν׾ -- ©øÂν׾¡@¡@ UG4 Laboratory of Ecological Genetics, Graduate School of Environmental Science, Hokkaido University, Sapporo, Japan.dB] ¥ÍºA¿ò¶Ç¬ã¨s«Ç,¤é¥»¥¾·E¥_®ü¹D¤j¾ÇÀô¹Ò¬ì¾Ç¬ã¨s©Ò&QwR ©©øÂν׾ -- ©øÂν׾¡@¡@ "dBh&V Abstract¤jºõ| ©©øÂν׾ -- ©øÂν׾¡@¡@ ]?(]j} The morphological diversity of insects is one of the most striking phenomena in biology.dYm}X ©øÂΫ¬ºAºØÃþ°Ï¤À¬O¦b¥Íª«¾Ç¸Ì¨ä¤¤¤@¶µ³Ì¤Þ¤Hª`·Nªº¦ÛµM²{¶H7J ©©øÂν׾ -- ©øÂν׾¡@¡@ +yQ Evolutionary modifications to the relative sizes of body parts, including the evolution of traits with exaggerated proportions,are responsible for a vast range of body forms.eW_+, ºt¤Æªº§ïÅܨìÓÅé¬ÛÃö¤Ø¤oªºÃö«Y,¥]§t¥H¸Ø±iªº¤jÃE¯S¼xªººt¤Æ¹Lµ{¬O¸ò¤j½d³òªº¨Å髬ºA¦³µÛ±K¤ÁªºÃö«Y.W\ ©©øÂν׾ -- ©øÂν׾¡@¡@ 5E@Ov Remarkable examples of an insect trait with exaggerated proportions are the mandibular weapons of stag beetles.G>KA1< ¸û·|Åý¤Hª`·N¨ìªºÄ´¦p¦³µÛ¸Ø±i¤ñ¨Ò©øÂίS¼x.§â¤jÃE·íªZ¾¹ªºÁê§ÎÂÎ8XT ©©øÂν׾ -- ©øÂν׾¡@¡@ 7b Male stag beetles possess extremely enlarged mandibles which they use in combat with rival males over females. As with other sexually selected traits,
¤½Áê§ÎÂξ֦³·¥¤jªº¤jÃE,¥Î¦b¹ï§Ü¥ÀÂΨ®Çªº¨ä¥L¤½Áê§ÎÂιï¤â¤W,·í¥H¤jÃE·í¦¨©Ê§O¿ï¾Üªº¤@ºØ¯S¼x®É,) stag beetle mandibles vary widely in size among males, and this variable growth results from differential larval nutrition.kS'I ¦b©Ò¦³¤½Áê§ÎÂΨ½¤jÃE¦³«Ü¼e¼sªºÅܤÆ,³oÓ¦hÅܤƪº¥Íªø²£¥Í©ó¨Ó¦Û¤£¦Pªº¥®ÂήɴÁ¾i¤ÀXH ©©øÂν׾ -- ©øÂν׾¡@¡@ y P However, the mechanisms responsible for coupling nutrition with growth of stag beetle mandibles (or indeed any insect structure) remain largely unknown.P^4 µM¦Ó,¥æ°t¾i¤Àªº²Õ¦¨»PÁê§ÎÂΤjÃE¦¨ªø(©Î¥ô¦ó©øÂε²ºc)ªºÃö«Y¤´¦s¦b«Ü¤jªº¥¼ª¾¼ÆVEQ}cJ ©©øÂν׾ -- ©øÂν׾¡@¡@ *Xf1Q Here, we demonstrate that during the development of male stag beetles (Cyclommatus metallifer),
juvenile hormone (JH) titers are correlated with the extreme growth of an exaggerated weapon of sexual selection.Ky ³o¸Ì,§ÚÌÃÒ¹ê...¦b¤½Áê§ÎÂΪºµo¨|´Á¶¡(¥H¬ü¥L¬°¨Ò)¥®ÂήɴÁªº¶Pº¸»X¶q¬O°Ï¤À©Ê§Oªº¸Ø±iªZ¾¹ªº·¥¤j¦a¦¨ªø¦³Ãö«Y)6YQ
We then investigate the putative role of JH in the development of the nutritionally-dependent, phenotypically plastic mandibles,l#H>de by increasing hemolymph titers of JH with application of the JH analog fenoxycarb during larval and prepupal developmental periods.
¦b¥®ÂΤεo¨|®É´Á,¹B¥Î¶Pº¸»Xªº¦¨¤ÀÀËÅ羯¤è¦¡¾aµÛ¼W¥[¥®ÂζPº¸»Xªº·»¾¯¶q,§ÚÌ©ó¬O¬ã¨s¥X¥®ÂζPº¸»X¦b¨Ì..¿à¾i¤Àªºµo¨|¦ÓÅãµÛ¦a§Î¦¨ªº¤jÃE©Ò§êºt¨¤¦â.W% ©©øÂν׾ -- ©øÂν׾¡@¡@ TB|d Increased JH signaling during the early prepupal period increased the proportional size of body parts, and this was especially pronounced in male mandibles,9s& enhancing the exaggerated size of this trait.8v<S ¦bªì´Áªº¥®ÂήɴÁ,¤H¤u¼W¥[ªº¥®ÂζPº¸»X«H¸¹¦]¤l·|¼W¤j¤F³¡¤À¨Å骺¤ñ¨Ò(¥i¯à«ü¤jÃE),¨Ã¥B³o¦b¤½ÂΤjÃE¯S¼x¤W´£¤É¤F¸Ø±iªº¤Ø¤o¤W¦³¯S§O¦a©úÅã.
The direction of this response is consistent with the measured JH titers during this same period. |Dfq?V (¤Wz)¤ÏÀ³¥X¨Óªºµ²ªG¸ò¦b¦P¤@®É´Á¶q´ú¥Xªº¥®ÂζPº¸»X¾¯¶q¬O¦³¤@PªºÃö«Y.,/0,g ©©øÂν׾ -- ©øÂν׾¡@¡@ 4 Combined,our results support a role for JH in the nutrition-dependent regulation of extreme mandible growth in this species.
ºî¦X¥H¤W,§Ú̱o¨ìªºµ²ªGÃÒ¹ê¤F¥®ÂζPº¸»X¬O³y¦¨³oª«ºØ²£¥Í·¥¤Ó¤jÃEªºÀç¾i¾¯²K¥[¤W¦³µÛ³o»ò¤@Ó§@¥Î.5&) ©©øÂν׾ -- ©øÂν׾¡@¡@ \ In addition, they illuminate mechanisms underlying the evolution of trait proportion, the most salient feature of the evolutionary diversification of the insects.h^T ¥t¥~,¥H¤W¤]©úÅãÃÒ©ú¤F(©øÂζPº¸»X)³o¬O¦b©øÂάɸ̳̩úÅã¶i¤Æªº¤ÀÃþ¤W,¯S¼x¤ñ¨Òµo®iªº¤@Óì²z(M< ©©øÂν׾ -- ©øÂν׾¡@¡@ c)R_ (Ó¤H»{¬°)²³æªº»¡,´N¬O¥Î¶Pº¸»X¾¯¶qªº²K¥[,Åý©øÂβ£¥Í¤F¤½¥À»P¤jÃE¤j¤pªº®t²§©ÊOC
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